MODIFIKASI ELEKTRODA KARBON DENGAN NIFEOX SEBAGAI PLATFORM SENSOR ELEKTROKIMIA UNTUK PENDETEKSIAN DOPAMIN

Anggita Putri Yasin; Anggara Budi Susila; Angga Hermawan

doi:10.21009/03.1201.FA36

Anggita Putri Yasin Program Studi Fisika, FMIPA Universitas Negeri Jakarta, Jl. Rawamangun Muka No. 01, Rawamangun 13220, Indonesia
Anggara Budi Susila Program Studi Fisika, FMIPA Universitas Negeri Jakarta, Jl. Rawamangun Muka No. 01, Rawamangun 13220, Indonesia
Angga Hermawan Pusat Riset Material Maju, Badan Riset Inovasi dan Nasional, Kawasan PUSPIPTEK, Gedung 440-442, Tangerang Selatan 15314, Indonesia

DOI: https://doi.org/10.21009/03.1201.FA36

Abstract

Abstrak

Pada penelitian ini, telah dilakukan fabrikasi dan karakterisasi elektroda sensor elektrokimia yang dilakukan untuk mendeteksi dopamin, salah satu biomarker penyakit parkinson, berbasis NiFeO_x menggunakan metode lelehan garam. Penelitian ini bertujuan untuk melihat pengaruh penggunaan lelehan garam KOH terhadap serta performa sensor elektrokimia dari NiFeO_x. Glassy carbon electrode (GCE) yang telah dimodifikasi dengan NiFeO_x (GCE@NiFeO_x) dikarakterisasi menggunakan teknik voltametri siklik untuk mendeteksi keberadaan dopamin. Pengukuran dilakukan dengan laju pemindaian 20-300 mV/detik padca konsentrasi dopamin 1 mmol didalam elektrolit 0.1 M PBS (Phosphate Buffered Saline) dengan rentang potensial -0.1- 0.4 V vs Ag/AgCl. Hasil penelitian secara voltametri siklik menunjukan bahwa sampel NiFeO_x sensitive terhadap molekul dopamin ditandai dengan arus puncak oksidasi sebesar 12 µA. Peningkatan ini disebabkan karena adanya peningkatan transfer elektron antara permukaan elektroda GCE@NiFeO_xdengan moekul dopamin dibandingkan dengan hanya menggunakan GCE.

Kata-kata kunci: NiFeO_x, dopamin, voltametri siklik, sensor elektrokimia.

Abstract

In this research, the fabrication and characterization of an electrochemical sensor electrode for the detection of dopamine, one of the biomarkers of Parkinson's disease, based on NiFeO_x using the molten salt method, have been conducted. This research aims to investigate the effect of using KOH molten salt on the phase and morphology characterization, as well as the electrochemical sensor performance of NiFeO_x. Cyclic voltammetry (CV) was performed to investigate electrochemical properties of the glassy carbon electrode (GCE) modified with NiFeO_x (GCE@ NiFeO_x) in detecting dopamine. CV measurement was carried out at a scan rate of 20-300 mV/s in a 1 mmol dopamine with 0.1 M PBS (Phosphate Buffered Saline) as a supporting electrolyte and potential range of -0.1 to 0.4 V vs Ag/AgCl. The results of CV analysis showed that the NiFeO_x sample was sensitive to dopamine molecules, as indicated by an oxidation peak current of 12 µA. This enhancement was attributed to the increased electron transfer between the GCE@ NiFeO_x electrode surface and dopamine molecules compared to using only GCE.

Keywords: NiFeO_x, dopamine, cyclic voltammetry, electrochemical sensor.

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