Imaging Performance Analysis of Green Mussel Shell-Derived Hydroxyapatite as a Sustainable Material for Bone Phantom Fabrication

Authors

  • Zhevanya Elisa Cleans Sihombing Medical Physics, Department of Physics, Faculty of Science and Mathematics, Mulawarman University, Samarinda 75123, Indonesia
  • Devina Rayzy Perwitasari Sutaji Putri Medical Physics, Department of Physics, Faculty of Science and Mathematics, Mulawarman University, Samarinda 75123, Indonesia
  • Erlinda Ratnasari Putri Medical Physics, Department of Physics, Faculty of Science and Mathematics, Mulawarman University, Samarinda 75123, Indonesia
  • Adrianus Inu Natalisanto Theoretical Physics, Department of Physics, Faculty of Science and Mathematics, Mulawarman University, Samarinda 75123, Indonesia
  • Sahara Hamas Intifadhah Material Physics, Department of Physics, Faculty of Science and Mathematics, Mulawarman University, Samarinda 75123, Indonesia

DOI:

https://doi.org/10.21009/SPEKTRA.112.03

Keywords:

Bone Phantom, Green Mussel Shell, Hydroxyapatite, Hounsfield Unit, Optical Density

Abstract

Bone phantom is a testing tool in radiology used to mimic the radiological properties of human bone tissue, with hydroxyapatite (HA) as one of its key mineral components. In this study, HA was synthesized from green mussel shells (Perna viridis) through two different shell preparation methods, namely HA 1 produced by calcining the shells in whole (uncrushed) form and HA 2 produced by calcining the shells after they had been crushed into powder. The mussel shell powder was first characterized using an Atomic Absorption Spectrophotometer (AAS) to determine its Calcium Oxide (CaO) content, followed by X-Ray Diffraction (XRD) after the HA synthesis process to confirm the formation of the HA material. Each HA powder was mixed with rice bran at ratios of 1:1, 1:2, and 2:1 to fabricate bone phantom prototypes, which were subsequently evaluated through X-Ray Radiography and CT-Scan imaging. Radiographic images were analyzed to obtain the mean gray value, hereafter reported as Optical Density (OD), while CT images were analyzed to obtain Hounsfield Unit (HU) values; both were compared against the reference radiodensity range of human bone. AAS analysis showed that the CaO content of the green mussel shells was 30.89%, and XRD confirmed the presence of HA diffraction peaks in a semi-crystalline phase for both HA 1 and HA 2. The highest OD value, 142.76, was obtained from HA 1 (whole-shell calcination) at a 1:1 ratio and 40 kVp, while the highest HU value, 2180.67 HU, was obtained from HA 2 (crushed-shell calcination) at a 1:1 ratio and 80 kV, a value that falls within the Hounsfield range of cortical bone (+700 to +3000 HU). These results indicate that the 1:1 ratio produced the most bone-equivalent radiodensity for each respective HA type, supporting the potential of green mussel shell-derived hydroxyapatite as a low-cost, sustainable material for bone phantom fabrication. This study contributes a reproducible, low-cost protocol for converting an underutilized marine waste stream into a bone-equivalent phantom material, together with quantitative OD and HU benchmarks across HA-type, composition-ratio, and exposure-voltage combinations, providing an empirical basis for the wider adoption of biogenic hydroxyapatite phantoms in radiological quality assurance and medical physics education.

References

B. Clarke, "Normal bone anatomy and physiology," Clin. J. Am. Soc. Nephrol., vol. 3, no. suppl. 3, pp. S131–S139, 2008, doi: 10.2215/CJN.04151206.

C. P. Putri Irawan, D. R. P. S. Putri, R. Munir, and Z. Sihotang, "Development of hydroxyapatite-based bone phantoms from blood cockle shells: Effect of material composition on X-ray attenuation," J. Penelit. Fis. dan Apl. (JPFA), vol. 15, no. 2, pp. 175–186, 2025, doi: 10.26740/jpfa.v15n2.p175-186.

A. W. Sari, A. Olivia Hidayati, D. Mahdo Hapiza, and A. Ridwan, "Pembuatan phantom radiologi menggunakan cangkang kerang sebagai pengganti bahan tulang," J. Online Phys., vol. 8, no. 2, pp. 50–55, 2023, doi: 10.22437/jop.v8i2.22303.

N. Dukov, V.-M. Valkova, M. Yordanova, V. Tsapaki, and K. Bliznakova, "Design and use of a custom phantom for regular tests of radiography apparatus: A feasibility study," J. Imag., vol. 10, no. 10, Art. no. 258, 2024, doi: 10.3390/jimaging10100258.

D. K. Jeong et al., "Effects of energy level, reconstruction kernel, and tube rotation time on Hounsfield units of hydroxyapatite in virtual monochromatic images obtained with dual-energy CT," Imag. Sci. Dent., vol. 49, no. 4, pp. 273–279, 2019, doi: 10.5624/isd.2019.49.4.273.

S. C. Bushong, Radiologic Science for Technologists: Physics, Biology, and Protection, 12th ed. Amsterdam, Netherlands: Elsevier, 2020.

C. A. Schneider, W. S. Rasband, and K. W. Eliceiri, "NIH Image to ImageJ: 25 years of image analysis," Nat. Methods, vol. 9, no. 7, pp. 671–675, 2012, doi: 10.1038/nmeth.2089.

T. Razi, P. Emamverdizadeh, N. Nilavar, and S. Razi, "Comparison of the Hounsfield unit in CT scan with the gray level in cone-beam CT," J. Dent. Res. Dent. Clin. Dent. Prospects, vol. 13, no. 3, pp. 177–182, Oct. 2019, doi: 10.15171/joddd.2019.028.

R. Gautreau and W. Savin, Modern Physics and Radiation Interaction. Boston, MA, USA: Addison-Wesley, 2006.

A. Prihanto, S. Muryanto, A. Sancho Vaquer, W. W. Schmahl, R. Ismail, J. Jamari, and A. P. Bayuseno, "In-depth knowledge of the low-temperature hydrothermal synthesis of nanocrystalline hydroxyapatite from waste green mussel shell (Perna viridis)," Environ. Technol., vol. 45, no. 12, pp. 2375–2387, 2024, doi: 10.1080/09593330.2023.2173087.

S. Patil, S. Thanikodi, S. Palaniyappan, J. Giri, A. O. Hourani, and M. Azizi, "Sustainable synthesis of marine-derived hydroxyapatite for biomedical applications: A systematic review on extraction methods and bioactivity," Adv. Manuf.: Polym. Compos. Sci., vol. 11, no. 1, Art. no. 2508547, 2025, doi: 10.1080/20550340.2025.2508547.

R. Ismail, T. Cionita, Y. L. Lai, D. F. Fitriyana, J. P. Siregar, A. P. Bayuseno, F. W. Nugraha, R. C. Muhamadin, A. P. Irawan, and A. E. Hadi, "Characterization of PLA/PCL/green mussel shells hydroxyapatite (HA) biocomposites prepared by chemical blending methods," Materials, vol. 15, no. 23, Art. no. 8641, 2022, doi: 10.3390/ma15238641.

J. S. R. de Andrade et al., "Sustainable valorization of mussel shell waste: Processing for calcium carbonate recovery and hydroxyapatite production," J. Funct. Biomater., vol. 17, no. 1, Art. no. 24, 2026, doi: 10.3390/jfb17010024.

S. V. Dorozhkin, "Bioceramics of calcium orthophosphates," Biomaterials, vol. 31, no. 7, pp. 1465–1485, 2010, doi: 10.1016/j.biomaterials.2009.11.050.

A. Yelten-Yilmaz and S. Yilmaz, "Wet chemical precipitation synthesis of hydroxyapatite from natural sources," Ceram. Int., vol. 44, no. 8, pp. 9703–9710, 2018, doi: 10.1016/j.ceramint.2018.02.201.

W. Mufida, A. P. Utami, and S. N. Dewi, "Pembuatan phantom radiologi berbahan dasar kayu lokal sebagai pengganti tulang manusia," J. Imejing Diagnostik (JImeD), vol. 6, no. 1, pp. 7–10, 2020, doi: 10.31983/jimed.v6i1.5404.

A. A. Oglat, "Comparison of X-ray films in term of kVp, mA, exposure time and distance using Radiographic Chest Phantom as a radiation quality," J. Radiat. Res. Appl. Sci., vol. 15, no. 4, Art. no. 100479, 2022, doi: 10.1016/j.jrras.2022.100479.

M. A. Farrukh, Principles of Atomic Absorption Spectrophotometry in Material Science. London, U.K.: Academic Press, 2024.

C. Anam, R. Amilia, A. Naufal, A. T. Maya, and Y. Dwihapsari, "The CT number uniformity and homogeneity: Which is better for detecting the beam hardening artefacts?," J. Teknol., vol. 87, no. 2, pp. 253–261, 2025, doi: 10.11113/jurnalteknologi.v87.21985.

Z. Zuliantoni, W. Suprapto, P. H. Setyarini, and F. Gapsari, "Extraction and characterization of snail shell waste hydroxyapatite," Results Eng., vol. 14, Art. no. 100390, 2022, doi: 10.1016/j.rineng.2022.100390.

A. R. Noviyanti, I. Rahayu, R. P. Fauzia, and Risdiana, "The effect of Mg concentration to mechanical strength of hydroxyapatite derived from eggshell," Arab. J. Chem., vol. 14, no. 4, Art. no. 103032, 2021, doi: 10.1016/j.arabjc.2021.103032.

J. T. Bushberg, J. A. Seibert, E. M. Leidholdt, and J. M. Boone, The Essential Physics of Medical Imaging, 3rd ed. Philadelphia, PA, USA: Lippincott Williams & Wilkins, 2012.

J. C. Elliott, Structure and Chemistry of the Apatites and Other Calcium Orthophosphates. Amsterdam, Netherlands: Elsevier, 1994.

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Published

2026-08-30

How to Cite

Sihombing, Z. E. C., Putri, D. R. P. S., Putri, E. R., Natalisanto, A. I., & Intifadhah, S. H. (2026). Imaging Performance Analysis of Green Mussel Shell-Derived Hydroxyapatite as a Sustainable Material for Bone Phantom Fabrication. Spektra: Jurnal Fisika Dan Aplikasinya, 11(2), 119–134. https://doi.org/10.21009/SPEKTRA.112.03

Issue

Vol. 11 No. 2 (2026): SPEKTRA: Jurnal Fisika dan Aplikasinya, Volume 11 Issue 2, August 2026

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Articles

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Copyright (c) 2026 Zhevanya Elisa Cleans Sihombing, Devina Rayzy Perwitasari Sutaji Putri, Erlinda Ratnasari Putri, Adrianus Inu Natalisanto , Sahara Hamas Intifadhah

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