IMPROVING THE ELASTIC MODULUS OF CLAY USING BIOSTIMULATED MICROBIALLY INDUCED CALCITE PRECIPITATION (MICP)

Authors

  • Fajri Hasan Nurrohman Civil Engineering, Faculty of Engineering, Kadiri University
  • Mahardi Kamalika Khusna Ali Civil Engineering, Faculty of Engineering, Kadiri University

DOI:

https://doi.org/10.21009/jpensil.v15i1.61448

Keywords:

Microbially Induced Calcite Precipitation (MICP), Biostimulation, Clay Soil (CL), Elastic Modulus, Unconfined Compressive Strength (UCS)

Abstract

Low-plasticity clay (CL) exhibits low stiffness and is easily deformable, thus requiring an efficient and environmentally friendly improvement method. Prior studies on Microbially Induced Calcite Precipitation (MICP) have predominantly emphasized improvements in soil compressive strength, whereas investigations addressing its effects on soil stiffness and elastic modulus remain relatively limited. Accordingly, the present study aims to evaluate the effectiveness of biostimulated MICP in enhancing the elastic modulus parameters (E₀ and Eₜ) of low-plasticity clay (CL). To achieve this objective, laboratory tests were conducted on soil specimens under two conditions, namely untreated samples and samples treated with an MICP solution, with incubation durations of 7 and 14 days. The Unconfined Compression Test (UCS) was conducted to obtain stress-strain relationships, which were then analyzed to determine the elastic modulus values. The results show that at 7 days of incubation, the MICP-treated soil exhibited increases of approximately 92% in E₀ and 90% in Eₜ compared to the untreated condition. With extended incubation to 14 days, the improvements became more pronounced, with E₀ increasing by approximately 104% and Eₜ by about 125% relative to untreated soil. The ANOVA results indicated that the differences between the untreated specimens and those treated with MICP were statistically significant, as evidenced by p-values below the 0.05 significance threshold. These findings demonstrate that the biostimulation process effectively enhances soil stiffness through interparticle calcite formation, providing a foundation for developing more efficient and eco-friendly soil stabilization methods applicable to infrastructure projects in tropical regions.

References

Al-Dahiree, O. S., Tokhi, M. O., Hadi, N. H., Hmoad, N. R., Ghazilla, R. A. R., Yap, H. J., & Albaadani, E. A. (2022). Design and Shape Optimization of Strain Gauge Load Cell for Axial Force Measurement for Test Benches. Sensors, 22(19), 1–19. https://doi.org/10.3390/s22197508

Arpajirakul, S., Pungrasmi, W., & Likitlersuang, S. (2021). Efficiency of microbially-induced calcite precipitation in natural clays for ground improvement. Construction and Building Materials, 282, 122722. https://doi.org/10.1016/j.conbuildmat.2021.122722

ASTM D2166. (2013). Standard Test Method for Unconfined Compressive Strength of Cohesive Soil 1. ASTM International, January, 1–7. https://doi.org/10.1520/D2166

ASTM D2487-17(2025). (2025). Practice for Classification of Soils for Engineering Purposes (Unified Soil Classification System). ASTM International, 17(Reapproved), 1–10. https://doi.org/10.1520/D2487-17R25.3

ASTM D4318-17. (2017). Pdf-Astm-D4318-17-Liquid-Limit-Plastic-Limit-and-Plasticity-Index-of-Soilspdf_Compress. ASTM International, 1–20. https://doi.org/10.1520/D4318-17E01.

ASTM D7928-21e1. (2021). D7928-21e1: Standard Test Method for Particle-Size Distribution (Gradation) of Fine-Grained Soils Using the Sedimentation (Hydrometer) Analysis. ASTM International Standard, 1–27. https://doi.org/10.1520/D7928-21E01

Chen, Y., Tan, L., Xiao, N., Liu, K., Jia, P., & Zhang, W. (2023). The hydro-mechanical characteristics and micro-structure of loess enhanced by microbially induced carbonate precipitation. Geomechanics for Energy and the Environment, 34, 100469. https://doi.org/10.1016/j.gete.2023.100469

Chittoori, B. C. S., Rahman, T., & Burbank, M. (2021). Microbial-Facilitated Calcium Carbonate Precipitation as a Shallow Stabilization Alternative for Expansive Soil Treatment. Geotechnics, 1(2), 558–572. https://doi.org/10.3390/geotechnics1020025

Chrysanthopoulos, E., & Kallioras, A. (2025). A Framework for Monitoring Soil Hydraulic Properties of Undisturbed Soil Samples and Modeling Unsaturated Zone Water Flow. Environmental Processes, 12(3). https://doi.org/10.1007/s40710-025-00791-1

Consoli, N. C., da Fonseca, A. V., Silva, S. R., Cruz, R. C., & Fonini, A. (2012). Parameters controlling stiffness and strength of artificially cemented soils. Geotechnique, 62(2), 177–183. https://doi.org/10.1680/geot.8.P.084

DeJong, J. T., Mortensen, B. M., Martinez, B. C., & Nelson, D. C. (2010). Bio-mediated soil improvement. Ecological Engineering, 36(2), 197–210. https://doi.org/10.1016/j.ecoleng.2008.12.029

Fan, W., Xiao, Y., Cao, B., Shi, J., Wu, H., & Shu, S. (2024). Comparison of bioaugmentation and biostimulation approaches for biocementation in soil column experiments. Journal of Building Engineering, 82(December 2023), 108335. https://doi.org/10.1016/j.jobe.2023.108335

Farichah, H., Hutama, D. A., & Solin, D. P. (2023). Evaluation of the Strength Characteristic of Soil Stabilized With Fly Ash. Jurnal PenSil, 12(3), 273–280. https://doi.org/10.21009/jpensil.v12i3.37489

Gowthaman, S., Kumamoto, Y., Nakashima, K., Takano, C., & Kawasaki, S. (2025). Proposing a new sustainable approach for sand improvement using biologically-derived calcium phosphate cement. Biogeotechnics, 3(4), 100135. https://doi.org/10.1016/j.bgtech.2024.100135

Graddy, C. M. R., Gomez, M. G., Dejong, J. T., & Nelson, D. C. (2021). Native Bacterial Community Convergence in Augmented and Stimulated Ureolytic MICP Biocementation. Environmental Science and Technology, 55(15), 10784–10793. https://doi.org/10.1021/acs.est.1c01520

Gupta, R. C. (2000). An approach for estimating deformation moduli from self-boring pressuremeter test data. Soils and Foundations, 40(1), 23–33. https://doi.org/10.3208/sandf.40.23

Hariprasad, C., Rajashekhar, M., & Umashankar, B. (2016). Preparation of Uniform Sand Specimens Using Stationary Pluviation and Vibratory Methods. Geotechnical and Geological Engineering, 34(6), 1909–1922. https://doi.org/10.1007/s10706-016-0064-0

Huang, H., Huang, M., & Ding, J. (2018). Calculation of Tangent Modulus of Soils under Different Stress Paths. Mathematical Problems in Engineering, 2018. https://doi.org/10.1155/2018/1916761

Huang, J., Wu, D., Wang, Z., Lu, N., Liu, S., & Lu, N. (2025). Experimental investigation on the construction waste recycled sand reinforced by soybean urease-induced calcium carbonate precipitation. November, 1–16. https://doi.org/10.3389/feart.2025.1687298

Islam, M. T., Chittoori, B. C. S., & Burbank, M. (2019). Evaluating the Applicability of Biostimulated Calcium Carbonate Precipitation to Stabilize Clayey Soils. Journal of Materials in Civil Engineering. https://doi.org/10.1061/(ASCE)MT.1943-5533.0003036

Kannan, K., Bindu, J., & Vinod, P. (2020). Engineering behaviour of MICP treated marine clays. Marine Georesources and Geotechnology, 38(7), 761–769. https://doi.org/10.1080/1064119X.2020.1728791

Kariminia, T., Rowshanzamir, M. A., Bak, H. M., Abtahi, S. M., Khoshghalb, A., Shahbodagh, B., Soleimanian-Zad, S., & Baghbanan, A. (2025). Enhancing the efficiency and sustainability of soil Bio-Cementation improvement using Persian gum. Scientific Reports, 15(1), 1–15. https://doi.org/10.1038/s41598-025-05607-8

Lehmann, P., Leshchinsky, B., Gupta, S., Mirus, B. B., Bickel, S., Lu, N., & Or, D. (2021). Clays Are Not Created Equal: How Clay Mineral Type Affects Soil Parameterization. Geophysical Research Letters, 48(20), 1–10. https://doi.org/10.1029/2021GL095311

Lv, Y., Wu, L., Duan, Z., Su, Y., & Zhang, D. (2025). Impact behavior and strain rate effects of artificial limestone by MICP. Biogeotechnics, 3(2), 100154. https://doi.org/10.1016/j.bgtech.2024.100154

Małkowski, P., Ostrowski, Ł., & Brodny, J. (2018). Analysis of Young’s modulus for Carboniferous sedimentary rocks and its relationship with uniaxial compressive strength using different methods of modulus determination. Journal of Sustainable Mining, 17(3), 145–157. https://doi.org/10.1016/j.jsm.2018.07.002

Nkalih, A. M., Pilate, P., Yongue, R. F., Njoya, A., & Fagel, N. (2018). Suitability of Foumban Clays (West Cameroon) for Production of Bricks and Tiles. Journal of Minerals and Materials Characterization and Engineering, 06(02), 244–256. https://doi.org/10.4236/jmmce.2018.62018

Obrzud, R. F., & Truty, A. (2018). The Hardening Soil Model - A Practical Guidebook. Z Soil.PC 100701 report. Zace Services Ltd, Software Engineering, 05, 205.

Ozcep, F. (2010). SoilEngineering: A Microsoft Excel® spreadsheet© program for geotechnical and geophysical analysis of soils. Computers and Geosciences, 36(10), 1355–1361. https://doi.org/10.1016/j.cageo.2010.01.015

Pakbaz, M. S., Ghezelbash, G. R., & Afzal, A. (2020). Sugarcane Molasses: A Cheap Carbon Source for Calcite Production in Different Class of Soils using Stimulation of Indigenous Urease-producing Bacteria. Geomicrobiology Journal, 37(3), 213–229. https://doi.org/10.1080/01490451.2019.1691684

Purba, D. A., Syahril, & Sagala, S. D. (2025). Improvement of Mechanical Properties of Clay With Calcite and Silica Fume: Unconfined Compressive Strength. Jurnal PenSil, 14(2), 199–211. https://doi.org/10.21009/jpensil.v14i2.54156

Rahman, M. M., Hora, R. N., Ahenkorah, I., Beecham, S., Karim, M. R., & Iqbal, A. (2020). State-of-the-art review of microbial-induced calcite precipitation and its sustainability in engineering applications. Sustainability (Switzerland), 12(15). https://doi.org/10.3390/SU12156281

Safi’i, A. D., Candra, A. I., & Ali, M. K. K. (2025). Increasing the Bearing Capacity of Square Foundations Based on Soybean Bio-Cementation. Jurnal PenSil, 14(3), 429–439. https://doi.org/10.21009/jpensil.v14i3.57680

Solaiman, M., Galib, A. R., Riam, S. Z., Sarwar Inam, A. K. M., & Tabassum, S. (2025). Real-time potentiometric sensing of soil nitrate: Depth-resolved monitoring across plant species with varying root structures. Computers and Electronics in Agriculture, 239(PA), 110929. https://doi.org/10.1016/j.compag.2025.110929

Song, C., Elsworth, D., Jia, Y., & Lin, J. (2022). Permeable rock matrix sealed with microbially-induced calcium carbonate precipitation: Evolutions of mechanical behaviors and associated microstructure. Engineering Geology, 304(April), 106697. https://doi.org/10.1016/j.enggeo.2022.106697

Thoriya, A., Rangwala, H., Kamati, D., & Vora, T. (2025). Seismic sensitivity analysis of plan-irregular RC buildings using statistical and machine learning approaches. Asian Journal of Civil Engineering. https://doi.org/10.1007/s42107-025-01471-z

Tiwari, N., Satyam, N., & Sharma, M. (2021). Micro-mechanical performance evaluation of expansive soil biotreated with indigenous bacteria using MICP method. Scientific Reports, 11(1), 1–12. https://doi.org/10.1038/s41598-021-89687-2

Tiwari, S. (2021). tiwari satyam 2021.

Urmi, Z. A., Saeidi, A., Yerro, A., & Chavali, R. V. P. (2023). Prediction of post-peak stress-strain behavior for sensitive clays. Engineering Geology, 323(June), 107221. https://doi.org/10.1016/j.enggeo.2023.107221

Wang, Z., Yang, T., Liu, Y., Jiang, Q., Shang, H., & Zheng, C. (2024). Montmorillonite combined with microbially induced carbonate precipitation for wind erosion control of bare surface soil in arid mining area. Process Safety and Environmental Protection, 187(February), 926–939. https://doi.org/10.1016/j.psep.2024.05.015

Won, J., Park, J., Kim, J., & Jang, J. (2021). Impact of particle sizes, mineralogy and pore fluid chemistry on the plasticity of clayey soils. Sustainability (Switzerland), 13(21). https://doi.org/10.3390/su132111741

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Published

2026-01-31

How to Cite

Nurrohman, F. H., & Ali, M. K. K. (2026). IMPROVING THE ELASTIC MODULUS OF CLAY USING BIOSTIMULATED MICROBIALLY INDUCED CALCITE PRECIPITATION (MICP). Jurnal Pensil : Pendidikan Teknik Sipil, 15(1), 66–77. https://doi.org/10.21009/jpensil.v15i1.61448