Analisis Kadar Kafein dalam Sampel Kopi Instan dan Minuman Berenergi Menggunakan High Performance Liquid Chromatography (HPLC)

Novita Cahyani Fitri; Yussi Pratiwi

doi:10.21009/JRSKT.092.04

Novita Cahyani Fitri Program Studi Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Negeri Jakarta, Gedung KH. Asj’arie, Jl. Rawamangun Muka, 13220, Jakarta, Indonesia..
Yussi Pratiwi Program Studi Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Negeri Jakarta, Gedung KH. Asj’arie, Jl. Rawamangun Muka, 13220, Jakarta, Indonesia.

DOI: https://doi.org/10.21009/JRSKT.092.04

Keywords: HPLC, kafein, kopi instan, minuman berenergi

Abstract

Abstrak

Analisis kadar kafein dalam sampel menggunakan HPLC telah dilakukan. Analisis ini bertujuan untuk menentukan kadar kafein yang ada dalam sampel berupa kopi instan dan minuman berenergi serta membandingkannya dengan persyaratan SNI yang ada. Metode yang digunakan meliputi pembuatan fasa gerak, preparasi larutan standar, serta preparasi sampel. Hasil yang didapatkan ialah kadar pada sampel-1, sampel-2, dan sampel-3 berturut-turut adalah sebesar 3,24256 mg/g, 3,07105 mg/g, dan 3,1861 mg/g. sedangkan pada sampel-4 dan sampel-5 sebesar 0,14149 mg/ml dan 0,1337 mg/ml dan semua sampel masih memenuhi SNI yang ada.

Kata kunci: HPLC, kafein, , kopi instan, minuman berenergi

Abstract

Analysis of caffeine content in samples using HPLC has been carried out. This analysis aims to determine the levels of caffeine present in the samples in the form of instant coffee and energy drinks and compare them with the existing SNI requirements. The methods used include preparation of the mobile phase, standard solution preparation, and sample preparation. The results obtained were the levels in sample-1, sample-2, and sample-3 respectively 3.24256 mg/g, 3.07105 mg/g, and 3.1861 mg/g. while in sample-4 and sample-5 it was 0.14149 mg/ml and 0.1337 mg/ml and all samples still met the existing SNI.

Keywords: caffeine, energy drink , HPLC, instan coffe

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