Validasi Metode Analisis Fosfor pada Sampel Daging dengan Pereduksi Hidrazin Sulfat menggunakan Spektrofotometri Uv-Vis
DOI:
https://doi.org/10.21009/JRSKT.101.03Keywords:
hidrazin sulfat, metode analisis fosfor, spektrofotomateri UV-VisAbstract
Abstrak
Pakan ternak yang biasanya dikonsumsi untuk menjaga nutrisi ternak mengandung fosfor. Fosfor dalam pakan ternak dapat menyebabkan akumulasi fosfor dalam daging ternak. Oleh karena itu, analisis fosfor secara berkala diperlukan untuk memastikan makanan tersebut aman untuk dikonsumsi. Analisis fosfor diukur dengan menggunakan metode spektrofotometri UV-Vis dengan menentukan kondisi optimum untuk analisis fosfor kemudian dilanjutkan validasi metode analisis yang terdiri dari parameter linearitas, sensitivitas, batas deteksi dan kuantitasi, akurasi dan presisi. Reaksi molibdenum biru menghasilkan senyawa kompleks fosfomolibdat dengan panjang gelombang 689 nm. Validasi metode analisis fosfat tereduksi hidrazin sulfat memiliki linearitas dengan nilai R2 = 0,9984 pada rentang 0 - 0,05 mg L-1 . Absorptivitas molar sebesar 7,9259 x 104 L mol-1 cm-1 . Nilai limit deteksi (LOD) sebesar 5,26 x 10-4 mg L-1 dan nilai limit kuantitasi (LOQ) sebesar 1,59 x 10-3 mg L-1 . Nilai presisi dinyatakan dalam persen simpangan baku relatif %RSD pada daging bebek, ayam dan sapi sebesar 1,33%, 1,34% dan 1,08%. Persen perolehan kembali yang diperoleh untuk setiap sampel daging berkisar antara 90% - 117%. Fosfat yang terkandung dalam daging bebek, ayam dan sapi tidak melebihi baku mutu menurut Persatuan Ahli Gizi Indonesia (PERSAGI).
Kata kunci: hidrazin sulfat, metode analisis fosfor, spektrofotomateri UV-Vis
Abstract
Animal feed which is usually consumed to maintain the nutrition of livestock contains phosphor. The phosphor in animal feed may cause an accumulated phosphor in meat. Therefore, a regular phosphor analysis is needed to make sure the food is safe. Phosphor analysis is measured by using spectrophotometry UV-Vis method by determining the optimum conditions for phosphorus analysis and then continued analysis validation which consists of linearity parameter, sensitivity, limit of detection and quantitation, accuracy, and precision. The reaction of blue molybdenum results in complex phosphomolybdate compound with a wavelength of 689 nm. The method validation of analysis of phosphate reduced hydrazine sulfate have a linearity of the method with R2=0.9984 in the range of 0 - 0.05 mg L-1 . The molar absorptivity of 7.9259 x 104 L mol-1 cm-1 . The value of limit of detection (LOD) of 5.26 x 10-4 mg L-1 and quantitation limit (LOQ) value of 1.59 x 10-3 mg L-1 . The precision values were expressed as percent of the relative standard deviations of %RSD in duck, chicken and cow of 1.33%, 1.34% and 1.08%. The percent recovery is obtained for each sample of meat ranged from 90% - 117%. Phosphate contained in duck, chicken and cow did not exceed the quality standard according to the Indonesian Nutritionist Association (PERSAGI).
Keywords: analysis fosfor method, hidrazin sulfat, spektrofotomateri UV-Vis
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