Development of a STEM-based worksheet on biotechnology to enhance creative thinking of biology students
DOI:
https://doi.org/10.21009/biosferjpb.64545Keywords:
21st-century Skills, Higher Order Thinking, Science Technology Engineering , MathematicsAbstract
This study aims to develop STEM-based student worksheet on environmental biotechnology material and analyze their validity, practicality, and effectiveness in improving students' creative thinking skills. This study uses the 4D model development method, which includes the define, design, develop, and disseminate stages, with 40 students from the Biology Study Program at Surabaya State University class of 2025 as the research subjects. The research instruments included expert validation sheets, student response questionnaires, activity observation sheets, and pretest and posttest tests of creative thinking skills. The data were analyzed using validity and practicality percentages and N-Gain scores to measure effectiveness. The results showed that the STEM-based student worksheet were highly valid with a validity percentage above 85% and highly practical based on student responses. The readability level was appropriate for students. The effectiveness of the student worksheet was demonstrated by an increase in the average creative thinking ability score from 68.75 on the pretest to 95.00 on the posttest with N-Gain values in the high to very high category. These findings indicate that the integration of the STEM approach through experimental activities and the use of technology is effective in training student creativity. This study recommends the use of STEM-based student worksheet as innovative teaching materials to support biotechnology learning in higher education.
References
Aguiar, A. A., & Calabrese, J. (2025). Broadening the perspective of traditional lecturing vs . active learning in introductory college biology. Discover Education, 4(278). https://doi.org/10.1007/s44217-025-00729-7
Alfiza, P. N., Susilawati, S., Syukri, M., Ridha, S., Umar, M. F., Hamid, A., Education, P., Kuala, U. S., & Aceh, B. (2024). An Innovative STEM-Based Worksheet to Enhance Scientific Literacy at Senior High School. Atlantis Press SARL. https://doi.org/10.2991/978-2-38476-216-3
Ameeque. (2026). Creative thinking, mathematics achievement , and well-being : A multilevel analysis of Spanish students. International Journal of Educational Research Open, 10, 100571. https://doi.org/10.1016/j.ijedro.2025.100571
Animasaun, D. A., Adedibu, P. A., Shkryl, Y., Emmanuel, F. O., Tekutyeva, L., & Balabanova, L. (2023). Modern Plant Biotechnology : An Antidote against Global Food Insecurity. Agronomy, 13(8), 1–26. https://doi.org/10.3390/agronomy13082038
Ansori, M. Z., Faiza, D., & Thamrin. (2024). Development of Digital Circuits Learning Media Using Articulate Storyline with the 4D Model. Journal of Hypermedia & Technology-Echanced Learning, 2(3), 338–351. https://doi.org/10.58536/j-hytel.v2i3.145
Anwar, S., Menekse, M., & Bryan, L. A. (2022). The effectiveness of an integrated STEM curriculum unit on middle school students ’ life science learning. National Science Foundation (NSF), July 2021, 1204–1234. https://doi.org/10.1002/tea.21756
Astawan, I. G., Suarjana, I. M., Werang, B. R., Asaloei, S. I., Sianturi, M., & Elele, E. C. (2023). STEM-Based Scientific Learning and Its Impact On Students’ Critical And Creative Thinking Skills: An Empirical Study. Jurnal Pendidikan IPA Indonesia, 12(3), 482–492. https://doi.org/10.15294/jpii.v12i3.46882
Ates, C. B., & Aktamis, H. (2024). Investigating the effects of creative educational modules blended with Cognitive Research Trust ( CoRT ) techniques and Problem Based Learning ( PBL ) on students ’ scientific creativity skills and perceptions in science education. Thinking Skills and Creativity, 51(September 2023), 101471. https://doi.org/10.1016/j.tsc.2024.101471
Choppin, J., Mcduffie, A. R., Drake, C., Davis, J., Choppin, J., Mcduffie, A. R., Drake, C., & The, J. D. (2020). The role of instructional materials in the relationship between the official curriculum and the enacted curriculum ABSTRACT. Mathematical Thinking and Learning, 00(00), 1–26. https://doi.org/10.1080/10986065.2020.1855376
Corr, R., & Mour, L. (2025). Creativity in higher education : A systematic literature review creativity in higher education. International Journal of Educational Research, 132(December 2023), 102613. https://doi.org/10.1016/j.ijer.2025.102613
Fior, G., Fonda, C., & Canessa, E. (2025). Hands-on STEM learning experiences using digital technologies. STEM Education, 5(2), 171–186. https://doi.org/10.3934/steme.2025009
Guo, P., Saab, N., Post, L. S., & Admiraal, W. (2020). A review of project-based learning in higher education : Student outcomes and measures. International Journal of Educational Research, 102(April), 101586. https://doi.org/10.1016/j.ijer.2020.101586
Gustati, Sriyunianti, F., Ferdawati, & Rissi, D. M. (2025). N-Gain Sebagai Alat Ukur Pemahaman Mahasiswa Pada Akuntansi Keuangan Lanjutan 1. Jurnal Ekonomi Dan Bisnis Dharma Andalas, 27(1), 11–24. https://doi.org/10.47233/jebd.v27i1.1763
Hattie, J., & Abad, L. G. (2025). The impact of teaching materials on instructional design and teacher development. Frontiers in Education, 10, 1577721. https://doi.org/10.3389/feduc.2025.1577721
Huda, A., Azhar, N., Anshari, K., Padang, U. N., Hartanto, S., & Kepulauan, U. R. (2020). Practicality and Effectiveness Test of Graphic Design Learning Media Based on Android. International Journal of Interactive Mobile Technologies (IJIM), 14(4), 192–203. https://doi.org/10.3991/ijim.v14i04.12737
Inggriyani, F., MZM, D., Gunawan, M., & Praja, A. L. (2022). Grafik Fry Dalam Mengukur Keterbacaan Wacana Buku Tematik Kelas IV Sekolah Dasar. Didaktik : Jurnal Ilmiah PGSD STKIP Subang, 08(01). https://doi.org/10.36989/didaktik.v8i1.305
Kahar, M. S., Syahputra, R., Arsyad, R. Bin, Nursetiawan, & Mujiarto. (2021). validity level of the developed Student Worksheets,. Eurasian Journal of Educational Research, 96, 14–29. https://doi.org/10.14689/ejer.2021.96.2
Karunarathne, W., & Calma, A. (2024). Assessing creative thinking skills in higher education : deficits and improvements. Studies in Higher Education, 5079(49), 1. https://doi.org/10.1080/03075079.2023.2225532
Kraus, E. B., Goecke, B., & Jaggy, A. (2026). The essence of creative thinking in PISA 2022 : An item-level machine learning approach to construct validity at the facet level $. Thinking Skills and Creativity, 61(October 2025), 102205. https://doi.org/10.1016/j.tsc.2026.102205
Kwon, H., & Lee, Y. (2025). A meta-analysis of STEM project-based learning on creativity. STEM Education, 5(2), 275–290. https://doi.org/10.3934/steme.2025014
Leedy, P. D., & Ormrod, J. E. (2015). Partictical Research. In J. W. Johnston (Ed.), Global Edition (Eleventh E). Kevin Davis.
Li, S., & Yu, S. (2025). Transforming higher education for the knowledge economy : Enhancing creative thinking and problem-solving skills through collaborative learning. Thinking Skills and Creativity, 57(October 2024), 101853. https://doi.org/10.1016/j.tsc.2025.101853
Mayer, R. E. (2021). Multimedia Learning (berilustra). Cambridge University Press.
Mayer, R. E. (2024). The Past , Present , and Future of the Cognitive Theory of Multimedia Learning. Educational Psychology Review, 36(1), 1–25. https://doi.org/10.1007/s10648-023-09842-1
Mendoza, N. V, & Sotomayor, T. (2023). Heliyon Effective teaching in computational thinking : A bias-free alternative to the exclusive use of students ’ evaluations of teaching ( SETs ). Heliyon, 9(8), e18997. https://doi.org/10.1016/j.heliyon.2023.e18997
Minatani, A., Supriyatin, & Rusdi. (2025). STEM e-worksheet innovation: Encouraging collaboration in excretory system learning. Biosfer: Jurnal Pendidikan Biologi, 18(2), 33–44. https://doi.org/10.21009/biosferjpb.52834
Mutlu-bayraktar, D., Cosgun, V., & Altan, T. (2019). Cognitive load in multimedia learning environments: A systematic review. In Computers & Education. Elsevier Ltd. https://doi.org/10.1016/j.compedu.2019.103618
Nadhiroh, N., & Latifah, S. (2020). Higher Order Thinking Skilss ( HOTS ) -Based Students ’ Worksheets In Thermodynamics Materials. Indonesian Journal of Science and Mathematics Education, 03(March), 87–95. https://doi.org/10.24042/ijsme.v3i1.6082
Nazzal, L. J., & Kaufman, J. C. (2020). The Relationship of the Quality of Creative Problem Solving Stages to Overall Creativity in Engineering Students. Thinking Skills and Creativity, 100734. https://doi.org/10.1016/j.tsc.2020.100734
Nur, S., & Mahmud, D. (2024). STEM service learning in higher education : A systematic literature review. EURASIA Journal of Mathematics, Science and Technology Education, 20(12). https://doi.org/10.29333/ejmste/15705
OECD. (2024). PISA 2022 Results Volume III : Creative Minds , Creative Schools- Factsheets: Indonesia. In OECD Publishing: Vol. III. https://doi.org/10.1787/765ee8c2-en
Pratama, F. Y., Mahardiani, L., & Bramastia. (2025). Research trends of creative thinking skill in science education journals in Indonesia : Design analysis and data analysis techniques. Journal of Environment and Sustainability Education, 3(1), 88–98. https://doi.org/10.62672/joease.v3i1.66
Purnomo, P. A., & Puspitawati, R. P. (2025). Pengembangan E-Modul Berbasis STEM Pada Materi Pencemaran Udara Untuk Melatihkan Kemampuan Berpikir Kreatif Siswa. Bioedu: Berkala Ilmiah Pendidikan Biologi, 14(3), 630–638. https://doi.org/10.26740/bioedu.v14n3.p630-638
Putri, R. Y., Puspaningtyas, N. D., & Hadi, W. (2024). Students’ Mathematical Creative Thinking Skills in Solving Pisa Problems: a Case in Indonesia. Kalamatika: Jurnal Pendidikan Matematika, 9(2), 233–249. https://doi.org/10.22236/kalamatika.vol9no2.2024pp233-249
Ramadhani, N. A., & Hardianti, R. D. (2025). Building Students’ Creative Thinking Skills in Science Learning : A Systematic Review of STEM-Based Approaches. Jurnal Paedagogy: Jurnal Penelitian Dan Pengembangan Pendidikan, 12(3), 831–841. https://doi.org/10.33394/jp.v12i3.15964
Retno, R. S., Purnomo, Hidayat, A., Mashfufah, A., & Umah, E. C. (2025). Students’ Creative Thinking in STEM Integrated Project-Based Learning (PjBL-STEM). Journal of Education Research and Evaluation, 9(1), 142–152. https://doi.org/10.23887/jere.v9i1.84704
Rets, I., Astruc, L., Coughlan, T., & Stickler, U. (2022). English for Speci fi c Purposes Approaches to simplifying academic texts in English : English teachers ’ views and practices. English for Specific Purposes, 68, 31–46. https://doi.org/10.1016/j.esp.2022.06.001
Riduwan, & Sunarto. (2019). Pengantar Statistika Pendidikan, Sosial, Ekonomi, Komunikasi, dan Bisnis 10th ed. In Alfabeta (Akdon).
Runco, M. A., & Abdulla, A. M. (2024). Interactions among dimensions of divergent thinking as predictors of creative activity and accomplishment. Thinking Skills and Creativity, 53(February), 101583. https://doi.org/10.1016/j.tsc.2024.101583
Sarıoğlan, A. B., & Özkaya, Ö. Ş. (2023). Web Integrated STEM Learning : Effects on Students ’ Academic Achievement , Creativity and Metacognitive Awareness. Journal of Science Learning, 6. https://doi.org/10.17509/jsl.v6i3.56477
Septiani, S., Reza, R., Akil, H., & Aziz, A. (2025). Jenis-Jenis Skala dan Teknik Skoring dalam Penilaian Psikomotorik pada Mata Pelajaran Pendidikan Agama Islam. Jurnal Manajemen Dan Pendidikan Agama Islam, 3(4), 21–36. https://doi.org/10.61132/jmpai.v3i4.1166
Sonthong, W., Intanin, A., & Sanpundorn, S. (2023). The Development of Science Learning Activities by Applying the STEM Education Model to Promote Student Creativity. International Journal of Research in STEM Education (IJRSE), 5(1), 55–66. https://doi.org/10.33830/ijrse.v5i1.1345
Sugiyono. (2015). Metode Penelitian Pendidikan (Pendekatan Kuantitatif, Kualitatif, dan RdanD). Alfabeta.
Tanujaya, B., Prahmana, R. C. I., & Mumu, J. (2022). Likert Scale in Social Sciences Research: Problems and Difficulties Benidiktus. FWU Journal of Social Sciences, 16(4), 89–101. http://doi.org/10.51709/19951272/Winter2022/7
Thornhill miller, B., Camarda, A., Mercier, M., Burkhardt, J., Morisseau, T., Bourgeois-bougrine, S., Vinchon, F., Hayek, S. El, Augereau-landais, M., Mourey, F., Feybesse, C., Sundquist, D., & Lubart, T. (2025). Creativity , Critical Thinking , Communication , and Collaboration : Assessment , Certification , and Promotion of 21st Century Skills for the Future of Work and Education. Journal of Intelligence Review, 11(3), 54. https://doi.org/10.3390/ jintelligence11030054
Tohari, B., & Rahman, A. (2024). Konstruktivisme Lev Semonovich Vygotsky dan Jerome Bruner : Model Pembelajaran Aktif dalam Pengembangan Kemampuan Kognitif Anak. Nusantara: Jurnal Pendidikan Indonesia, 4(1). https://doi.org/10.14421/njpi.2024.v4i1-13
Wahdaniyah, N., & Agustini, R. (2023). Analysis of Effectiveness PBL-STEM to Improve Student ’ s Critical Thinking Skills. IJORER : International Journal of Recent Educational Research, 4(3), 369–382. https://doi.org/10.46245/ijorer.v4i3.312
Widodo, S. A., Hidayat, W., Ekawati, R., Irfan, M., & Maarif, S. (2025). The Importance of Creating Mathematical Worksheets and Their Impact on Critical and Creative Thinking Skills. European Journal of STEM Education, 10(1), 1–16. https://doi.org/10.20897/ejsteme/17487
Winaryati, E., H. Iksan, Z., Amoah Abd Rauf, R., Budiono, I. Kusumaningrum, W., Salaffudin, A., Heryani, D., Aditama, M. G., Nurdiana, L., & Kurniawan, J. (2025). Evaluation of the exploration of four character skills (4Cs): implementation of STEM-integrated PjBL through lesson study in school communities. International Journal for Lesson and Learning Studies, 15(1), 1–17. https://doi.org/10.1108/IJLLS-03-2025-0091
Zhang, S., Jiang, Q., Xiong, W., Fan, J., & Zhao, W. (2026). Investigating the factors influencing secondary school students ’ creative thinking based on the opportunity – propensity framework : Evidence from high-performing countries and regions. Thinking Skills and Creativity, 60(July 2025), 102067. https://doi.org/10.1016/j.tsc.2025.102067
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