Fasting Total Cholesterol with HIIT: Acute Response 20 Minutes After a Single 20-Minute HIIT Ergocycle Session in Young Men
DOI:
https://doi.org/10.21009/GJIK.173.04Keywords:
HIIT ergocycle, total cholesterol, acute execise, overnight fasting, capillary point-of-care deviceAbstract
This study aimed to determine whether total cholesterol levels differed between 10 minutes before exercise and 20 minutes after a single session of high-intensity interval training (HIIT) performed on an ergocycle under overnight fasting conditions. This quantitative study employed a one-group pretest–posttest design. The participants were 41 healthy male university students aged 20–21 years with a body mass index of 20–23.9 kg/m² who met the inclusion criteria. All participants underwent overnight fasting for at least 8 hours, with water intake permitted, and avoided strenuous physical activity for 48 hours before testing. Total cholesterol was measured using an EasyTouch point-of-care device from capillary blood samples collected before and after a 20-minute HIIT ergocycle session. Data were analyzed using the Shapiro–Wilk normality test and a paired-samples t-test, with statistical significance set at p < 0.05. The results showed that 97.6% of participants experienced a decrease in total cholesterol, with mean values declining from 183.93 ± 12.10 mg/dL before exercise to 168.78 ± 12.77 mg/dL after exercise. The difference was statistically significant, t(40) = −13.43, p < 0.001, with a large effect size (Cohen’s d<sub>z</sub> = −2.10). In conclusion, a single HIIT ergocycle session was associated with an acute reduction in measured total cholesterol concentration during early recovery under overnight fasting conditions.Downloads
References
Alansare, A., Alford, K., Lee, S., Church, T., & Jung, H. C. (2018). The effects of high-intensity interval training vs. Moderate-intensity continuous training on heart rate variability in physically inactive adults. International Journal of Environmental Research and Public Health. https://doi.org/10.3390/ijerph15071508
Andi Makbul Aman. (2021). Pengelolaan Dislipidemia Di Indonesia 2021. In PB Perkeni.
Bjerre-Bastos, J. J., Sejersen, C., Bihlet, A. R., Secher, N. H., Mackey, A. L., Kitchen, C. C., Drobinski, P., Thudium, C. S., & Nielsen, H. B. (2022). An Estimate of Plasma Volume Changes Following Moderate-High Intensity Running and Cycling Exercise and Adrenaline Infusion. Frontiers in Physiology, 13, 948087. https://doi.org/10.3389/fphys.2022.948087
Costa, R. R., Buttelli, A. C. K., Fagundes, A. de O., Fonseca, G. A., Pilla, C., Barreto, M. F., Viero, P. A., da Rocha, V. de M. B., Alberton, C. L., & Kruel, L. F. M. (2020). The beneficial effects of a water-based aerobic exercise session on the blood lipids of women with dyslipidemia are independent of their training status. Clinics (Sao Paulo, Brazil), 75. https://pubmed.ncbi.nlm.nih.gov/32130352/
Ercan, Ş., & Manav, A. (2025). Impact of fasting duration on LDL cholesterol concentrations estimated by the Friedewald, Martin-Hopkins, and Sampson/NIH equations. Biochemia Medica, 35(2), 235–248. https://doi.org/10.11613/BM.2025.020704
Kazeminasab, F., Rafiee, P., Miraghajani, M., Santos, H. O., Symonds, M. E., & Rosenkranz, S. K. (2025). The effects of acute bouts of exercise in fasted vs. fed states on glucose and lipid metabolism in healthy adults: A systematic review and meta-analysis of randomized clinical trials. Clinical Nutrition ESPEN, 66, 320–331. https://doi.org/10.1016/j.clnesp.2025.02.002
Kim, H. N., & Yoon, S. Y. (2021). Simultaneous point-of-care testing of blood lipid profile and glucose: Performance evaluation of the GCare Lipid Analyzer. Journal of Clinical Laboratory Analysis, 35(12). https://doi.org/10.1002/jcla.24055
Mutepfa, C. C., Hicks, T. P., Winter, A., Dickinson, R. E., Williams, C., Harrison, N., Chidanyika, J., Newton, J. L., Jones, W. S., & Suklan, J. (2025). Can we trust published evidence on point-of-care tests for cholesterol? A rapid review. BMJ Open, 15(3). https://doi.org/10.1136/bmjopen-2023-080726
Racil, G., Chelly, M. S., Coquart, J., Padulo, J., Teodor, D. F., & Russo, L. (2023). Long- and Short-Term High-Intensity Interval Training on Lipid Profile and Cardiovascular Disorders in Obese Male Adolescents. Children 2023, Vol. 10, Page 1180, 10(7), 1180. https://doi.org/10.3390/children10071180
Robert Lourdes, T. G., Chong, Z. L., Saminathan, T. A., Abd Hamid, H. A., Mat Rifin, H., Wan, K. S., Ab Majid, N. L., Yoga Ratnam, K. K., Riyadzi, M. R., Ismail, H., Alias, N., & Mohd Yusoff, M. F. (2024). Diagnostic accuracy of Cardiochek® PA point-of-care testing (POCT) analyser with a 3-in-1 lipid panel for epidemiological surveys. Lipids in Health and Disease , 23(1). https://doi.org/10.1186/s12944-024-02270-2
Stankovic, M., Čaprić, I., Pezelj, L., Biševac, E., Mekić, R., Zećirović, A., Salihagić, Z., Ajdinović, A., & Jelaska, I. (2025). High-Intensity Interval Training (HIIT): Impact of Duration on Body Composition, Cardiometabolic Health, and Aerobic Capacity in Adolescent Women. Metabolites 2025, Vol. 15, Page 623, 15(9), 623. https://doi.org/10.3390/metabo15090623
Vigriawan, G. E., Putri, E. A. C., Rejeki, P. S., Qurnianingsih, E., Kinanti, R. G., Mohamed, M. N. A., & Herawati, L. (2022). High-intensity interval training improves physical performance without C-reactive protein (CRP) level alteration in overweight sedentary women. Journal of Physical Education and Sport, 22(2), 442–447. https://doi.org/10.7752/jpes.2022.02055
Vigriawan, G. E., Raharjo, S., Andiana, O., Yunus, M., Pribadi, H. P., Abdullah, A., Sugiarto, D., Yuniarto, A., Kusuma Bakti, F. R. F., Ayubi, N., & Airlangga Daulay, D. A. (2024). LOWERING CHOLESTEROL WITH HIIT: AN EFFECTIVE SOLUTION FOR OVERWEIGHT WOMEN. Gladi : Jurnal Ilmu Keolahragaan, 15(4), 483–492. https://doi.org/10.21009/gjik.154.05
Yamaner, E., Turğut, T., Aksoy, A., Demirkıran, B., Uçar, M. A., Başoğlu, B., Çamiçi, F., Yanar, M. S., Bülbül, A., Koç, A. F., Ceylan, T., Ceylan, L., & Küçük, H. (2025). Impact of an 8-week high-intensity bodyweight interval training on body composition and blood lipid metabolism in young women with overweight. Frontiers in Public Health, 13, 1578569. https://doi.org/10.3389/fpubh.2025.1578569
Yu, S., Sun, H. Y., & Yoo, B. (2023). Performance evaluation of Barozen Lipid Plus for point-of-care testing of lipid profiles: a method comparison study. Journal of Yeungnam Medical Science, 40, S73–S80. https://doi.org/10.12701/jyms.2023.00528
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