HUBUNGAN EKSPRESI miRNA-21 DENGAN TARGET PDCD4 DAN miRNA-143 DENGAN TARGET BLC-2 PADA KASUS KANKER SERVIKS

  • Nikki Aldi Massardi Universitas Bengkulu
  • Soemanadi Obstetri dan Ginekologi Rumah Sakit Kanker Dharmais
  • Beti Ernawati Dewi Departemen Mikrobiologi, Universitas Indonesia
  • Dewi Wulandari Departemen Patologi Klinik, Universitas Indonesia
  • Eduardus Gilang Putra Kekhususan Onkologi, Magister Ilmu Biomedik FKUI
Keywords: miRNA-21, miRNA-143, PDCD4, Bcl-2, Cervical cancer

Abstract

A study has been carried out to compare the expression of miRNA-21 and miRNA-143 with target mRNA PDCD4 and BCL-2 in cases of early-stage cervical cancer compared to advanced stages. These data can be used as information for the development of non-invasive cervical cancer prognostic methods. The study was conducted by taking serum samples and exfoliativecell samples from normal subjects and subjects with cervical cancer detection and then analyzed using qRT-PCR. Samples of cervical cancer patients consisting of 15 subjects and 4 normal subjects were used to obtain relative quantity values for the expression of miRNA-21, miRNA-143, mRNA PDCD4 and BCL-2. In the study of miRNA-21, there was no significant difference in determining cervical cancer with p>0.05 using the t-test (0.594) and a significant relationship was found between miRNA 21 and PDCD4 with a strong correlation (p<0.05, r = -0.563; Pearson). The relationship between miRNA-143 and the Bcl-2 target gene in this study showed a weak and insignificant correlation (r = -0.101; p > 0.05; Pearson). There was a significant relationship between miRNA-21 and PDCD4 in early-stage and advanced-stage samples, while the relationship between miRNA-143 and the Bcl-2 mRNA target gene showed no significant correlation.

Published
2023-07-31
How to Cite
Massardi, N. A., Soemanadi, Dewi, B. E., Wulandari, D., & Putra, E. G. (2023). HUBUNGAN EKSPRESI miRNA-21 DENGAN TARGET PDCD4 DAN miRNA-143 DENGAN TARGET BLC-2 PADA KASUS KANKER SERVIKS. Bioma, 19(1), 52 - 61. https://doi.org/10.21009/Bioma19(1).6
Section
Articles