Bioprospecting secondary metabolites from Streptomyces sp. HPM-9-01/B9-A from South Kalimantan as a potential antibiotic candidate

Dwi Ariyanti; Maya Fitriana; Ali Budhi Kusuma; Matin Nuhamunada; Nurul Izzati; Aulia Reski Widyaningrum

doi:10.21009/bioma.v22i1.60548

Authors

Dwi Ariyanti Universitas Teknologi Sumbawa
Maya Fitriana Universitas Teknologi Sumbawa
Ali Budhi Kusuma Universitas Teknologi Sumbawa
Matin Nuhamunada Universitas Gadjah Mada
Nurul Izzati Universitas Teknologi Sumbawa
Aulia Reski Widyaningrum Synthetic Biology Indonesia

DOI:

https://doi.org/10.21009/bioma.v22i1.60548

Keywords:

actinobacteria, Streptomyces sp., South Kalimantan, antibiotic candidate

Abstract

Actinobacteria of the genus Streptomyces are widely recognised as a major reservoir of antibiotics. These bacteria occur abundantly in diverse environments, including soil, freshwater, and marine ecosystems. In this study, we employed isolation, characterisation, and analytical approaches encompassing morphological, genomic, and metabolomic techniques to investigate the potential of isolate HPM-9-01/B9-A, obtained from a pine forest in Mentaos, South Kalimantan, as a candidate for antibiotic production. Morphological characterisation combined with 16S rRNA sequencing identified the strain as Streptomyces albulus. Antibacterial assays demonstrated inhibitory activity against five pathogenic bacteria, with a strong inhibition zone of 16.75 ± 2.50 mm observed for Salmonella typhimurium. LC-MS analysis, supported by secondary metabolite identification using MassLynx and ChemSpider, revealed three major compounds: C₁₀H₁₆N₇O₂, C₁₂H₁₈NO₃, and C₁₇H₂₁N₄O₅. These metabolites are predicted to possess antibacterial and neuroactive properties. Collectively, these findings highlight the promising potential of Streptomyces sp. isolate HPM-9-01/B9-A as a source of novel antibiotic compounds.

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