DETERMINASI PEMBERIAN MINYAK TRANS TERHADAP STRES OKSIDATIF PADA TIKUS GALUR WISTAR DENGAN MALONDIALDEHIDA PANKREAS SEBAGAI INDIKATOR

Authors

  • Maharja Mawali Fakultas Kedokteran Hewan IPB
  • Koekoeh Santoso Fakultas Kedokteran Hewan IPB
  • Nastiti Kusumorini Fakultas Kedokteran Hewan IPB
  • Aryani Sismin Satyaningtijas
  • Atin Supiyani Jurusan Biologi FMIPA Universitas Negeri Jakarta (UNJ). Jl. Pemuda No. 10 Rawamangun, Jakarta Timur. 13220. Indonesia

DOI:

https://doi.org/10.21009/Bioma11(2).10

Keywords:

trans fatty acids, oxidative stress, malondialdehyde, Wistar rats, palm oil

Abstract

ABSTRACT

Trans fatty acids (TFAs) are the entire unsaturated fatty acids containing one or more double bonds isolated in trans configuration believed as predisposing factor of oxidative stress, excluding conjugated double bond fatty acids. Malondialdehyde (MDA) is a product of lipid peroxidation used as oxidative stress marker. The objective of this study was to see the correlation between TFAs containing-oil (consists of 3-6% TFAs) intake at various administration doses and pancreatic MDA levels in Wistar rats. Twelve rats were grouped according to administration doses (20%, 40%, 60% of given feed total energy and control group). TFAs containing-oil was administered orally once a day for 70 days at given doses by force feeding. Pancreatic MDA were measured using TBARS assay adapted from Singh et al. (2006). Increase of TFAs  containing-oil  administration  dose  caused  insignificant  increase  of  pancreatic MDA level.

 

Keywords: trans fatty acids, oxidative stress, malondialdehyde, Wistar rats, palm oil

Downloads

Published

2015-05-09

How to Cite

Mawali, M., Santoso, K., Kusumorini, N., Satyaningtijas, A. S., & Supiyani, A. (2015). DETERMINASI PEMBERIAN MINYAK TRANS TERHADAP STRES OKSIDATIF PADA TIKUS GALUR WISTAR DENGAN MALONDIALDEHIDA PANKREAS SEBAGAI INDIKATOR. Bioma, 11(2), 201–211. https://doi.org/10.21009/Bioma11(2).10

Issue

Section

Articles

Most read articles by the same author(s)